منابع مشابه
Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
متن کاملLetter to editor " Applying High-quality DNA Melting Curve Analysis in Identifying Staphylococcus aureus and Methicillin-resistant Strains "
Letter to editor " Applying High-quality DNA Melting Curve Analysis in Identifying Staphylococcus aureus and Methicillin-resistant Strains " Ramezan Ali Ataee Professor, Department of Medical Microbiology, Faculty of Medicine, Hospital Research Development Committee, Applied Microbiology Research Center, System Biology, Poisoning Institute, Baqiyatallah University of Medical Sciences, T...
متن کاملIn-tube DNA methylation profiling by fluorescence melting curve analysis.
BACKGROUND Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a two-step procedure, comprising initial PCR amplification and subsequent product analysis in separate operations that usually require manual transfer. These methods generally provide information about methylation of only a few CpG dinucleotides within the target sequence. METHODS An in-tube methylation assay i...
متن کاملRapid competitive PCR using melting curve analysis for DNA quantification.
A rapid competitive PCR method was developed to quantify DNA on the LightCycler. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR Green I. Specific hybridization probes are not required. Heterologous internal standards sharing the same primer binding sites and having different melting temperatures to the natural PCR pr...
متن کاملThe Melting Curve and Premelting of MgO
The melting curve for MgO was obtained using molecular dynamics and a non-empirical, many-body potential. We also studied premelting effects by computing the dynamical structure factor in the crystal on approach to melting. The melting curve simulations were performed with periodic boundary conditions with cells up to 512 atoms using the ab-initio Variational Induced Breathing (VIB) model. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.1.101